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*   Direct Infusion Algorithm
The Direct Infusion algorithm is a spectral tree construction utility. Use this algorithm to create one or more spectral trees from a single raw file by reading various MSn scans acquired in one run and constructing a tree according to their MS stage and precursor ion m/z values.
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To run the Direct Infusion algorithm
1.
Click the Components Detection & Spectra Deconvolution button, , and choose Direct Infusion.
The Direct Infusion Spectral Tree Construction dialog box opens.
2.
3.
Click Calculate.
4.
Direct Infusion Spectral Tree Construction dialog box
Specifies the MS stage that should only have a single precursor. The value determines the MS stage for a tree branch division. When you use the default value of 1, the algorithm creates a single tree from all the scans in a run (file). When you use a value of 2, and the analyzed run contains MS/MS spectra with different precursor m/z values, the algorithm creates separate trees for each MS/MS scan with a unique precursor ion m/z value.
Adds actual scans in the stage above the level set in Minimal Tree Depth to the resulting spectral trees. When you clear this option, the spectra above the stage set in Minimal Tree Depth contain spectra with a single peak equal to the precursor ion of the product spectra.
When selected, the software calculates the ion profile (envelope) for each tree. You can then preview scans that belong to a particular tree.
Specifies only a part of a chromatogram to be analyzed (time range), which can speed up your work. In this case, the algorithm ignores regions outside of the specified range.
m/z Range
Determines m/z value range for analysis. Ignores other spectral regions. This feature is useful when processing large chromatograms.
Opens the Save Direct Infusion Parameters File dialog box where you can save your current parameters to a Direct Infusion parameters file (.infusion.par).
Opens the Open Direct Infusion Parameters File dialog box where you can choose a Direct Infusion parameters file (.infusion.par) to load.


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Related Topics:
  JCD Algorithm
  RCD Algorithm
  TECD Algorithm
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